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HCA Pre-Conference Web Seminar
December 3, 2008, 10:00am – 12:30pm (EST)
Supplement HCA 2009 with a virtual forum and access new presentations!
Please join us for live interactive web seminars from the comfort of your office and learn about cutting-edge research and technology in HCA. No costs or hassles of travel, no time away from the office!
While the web seminars cannot match the level of networking, scope of scientific content, and hands-on nature of the trade show during the annual HCA meeting, they are meant to provide added value by giving you access to speakers who are not able to attend the annual meeting and to keep you updated on the latest developments.
Also available on-demand! If you have a conflict, simply sign-up, download after the webinar and view at your convenience.
10:00-10:10 Chairperson’s Opening Remarks
10:10-10:35 High-Content Screening as a Tool for Systems Biology
James G. Evans, Ph.D., Research Scientist, Computational and Systems Biology Initiative, Massachusetts Institute of Technology
Automated imaging and analysis provides a wealth of data from which information and ultimately, biological knowledge may be built. However, the scale and complexity of imaging-derived data often deters its use in high throughput screening. Systems biology aims to use imaging-derived data to develop quantitative models of dynamic multi-scalar biological systems and thus its progress depends on efficient mining and modeling of these large and expanding datasets.
Sponsored by 
10:35-10:45 Columbus Sets the Standard for Image Data Management
Martin Daffertshofer, Ph.D., Global Product Manager, Software, PerkinElmer
Columbus™ has been developed to solve the problem of HCS data management, providing all the tools required to archive, manage, retrieve and protect images and analyzed results. Designed as a partner product for the Opera™, PerkinElmer’s premier confocal microplate imaging reader, the system has the added benefit of full compatibility with a wide range of image file formats. This means that Columbus can be used to archive and manage images from confocal and standard research microscopes, and can therefore act as a convenient central repository for all your image data. Columbus utilizes the OMERO server, developed by the Open Microscopy Environment (OME), a multi-site collaborative effort among academic laboratories and a number of commercial entities that produces open tools to support data management for biological light microscopy.
10:45-11:10 High-Content Characterization of Phenotype and Physiology in Primary Neurons and Neural Stem Cell Cultures
Myles Fennell, Ph.D., Principal Research Scientist, Neuroscience, Bristol-Myers Squibb Co.
11:10-11:20 Sponsored Presentation (Opportunity Available.)
Contact Katelin Fitzgerald, Manager, Business Development, at 781-972-5458 or
kfitzgerald@healthtech.com.
11:20-11:45 Single Cell Cytometry Can Identify Fundamental Discordances in Cell Biology Studies Used for Basic Biology and Drug Discovery
Steven Haney, Ph.D., Senior Principal Scientist, Biological Profiling/Target Generation Unit, Bioinnovation and Biotherapeutics Center, Pfizer Research Technology Center
The ability to measure events at the single cell level can easily demonstrate that, despite the efforts to prepare robust and homogenous cell lines as disease models, the inherent variability can be wide. Furthermore, the analysis of an experiment at the single cell level, measuring and displaying the data for a sample as a population of cells, rather than an average of all the wells, can identify problems in basic assumptions about the design of the experiment. This seminar will present both basic examples of the difference between measuring events as averages and as populations, and the effect this can have on interpreting perturbations in signaling pathways.
11:45-11:55 Sponsored Presentation (Opportunity Available.)
Contact Katelin Fitzgerald, Manager, Business Development, at 781-972-5458 or
kfitzgerald@healthtech.com.
11:55-12:20 Application of High-Content Analysis to Genome-Wide RNAi Screens
Tiao Xie, Ph.D., Image Analyst, Department of Systems Biology/ICCB-Longwood, Harvard Medical School
The ICCB-Longwood Screening Facility, located at Harvard Medical School, serves academic researchers in carrying out high-throughput screens of small molecule and RNAi libraries. Among the screens at ICCB-L, imaging has been a popular readout due to its information rich nature. Efficiently quantitating data from the hundreds of thousands of images is a challenge and requires automated analysis algorithms. Straightforward readouts such as counting cells, monitoring mitotic index, and scoring gene/protein expression can be handled by commercially available software packages. However, many assays employ morphology-based image analysis, which generally requires highly customized analysis solutions. This talk will present morphology-based image analyses carried out for two genome wide RNAi screens. One screen identified siRNA enhancers and suppressors of treatment with anti-mitotic drug. The other screen, in the area of neurobiology, examined cell response to an important developmental stimulus. Both analyses were done using the CellProfiler Image Analysis package along with Matlab.
12:20-12:30 Closing Remarks
For more information, please contact:
Julia Boguslavsky, Executive Director, Conferences
E-mail: juliab@healthtech.com
For sponsorship information, please contact:
Katelin Fitzgerald, Manager, Business Development
Phone: 781-972-5458
E-mail: kfitzgerald@healthtech.com
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